Ausencia de efecto antiadipogénico en pez cebra de los polifenoles de extracto de hueso de aceituna

  1. Veciana Galindo, Carmen
  2. Torro Montell, Luís
  3. Sirvent Segura, Eliana
  4. Palazón-Bru, Antonio
  5. Rizo-Baeza, María Mercedes
  6. Gil-Guillén, Francisco Vicente
  7. Cortés-Castell, Ernesto
Journal:
Journal of Negative and No Positive Results: JONNPR

ISSN: 2529-850X

Year of publication: 2016

Volume: 1

Issue: 4

Pages: 138-141

Type: Article

DIALNET GOOGLE SCHOLAR lock_openDialnet editor

More publications in: Journal of Negative and No Positive Results: JONNPR

Abstract

Background and aim. Olive polyphenols have anti-inflammatory activity, prevent apoptosis by oxidative stress, promote neuroprotection and neurodevelopment, and have antiadipogenic effect in different cell models. The aim of this study was to analyze the possible antiadipogenic effect of olive polyphenols in zebrafish, measuring weight gain, cholesterol, triglycerides and fatty acids. Material and methods. Fertilized eggs were used and incubated in well plates with 26±1°C for 72 hours: Control group in water with dimethyl sulfoxide (0.1%); Intervention group, like the control group, but adding polyphenolic extract (concentration: 100 mg/l). After incubation we proceeded to measure body mass of dry larvae, proteins, total cholesterol, triglycerides and fatty acids. Results and discussion. No differences were shown between control and intervention group in all the analyzed parameters. These results were inconclusive possibly because we did not take into account that adipose tissue appears in zebrafish within 120 hours postfertilization. This opens new perspectives to study our extract, as we showed its antiadipogenic effectiveness in the differentiation of mouse fibroblasts to adipocytes. Conclusion. New studies should be performed in order to confirm or exclude the effect of olive polyphenols on lipid metabolism. These studies should take into account normal physiological conditions of the animal model chosen.

Bibliographic References

  • Hsu CL, Yen GC. Phenolic Compounds: Evidence for Inhibitory Effects against Obesity and Their Underlying
  • Molecular Signalling Mechanisms. Mol Nutr Food Res 2008; 52:53-61.
  • Wolfram S, Wang Y, Thielecke F. Anti-obesity effects of green tea: from bedside to bench. Mol Nutr Food Res
  • ; 50:176-87.
  • Rayalam S, Della-Fera MA, Baile CA. Synergism between resveratrol and other phytochemicals: implications for
  • obesity and osteoporosis. Mol Nutr Food Res 2011; 55:1177-85.
  • Lasa A, Churruca I, Eseberri I, Andrés-Lacueva C, Portillo MP. Delipidating effect of resveratrol metabolites in
  • T3-L1 adipocytes. Mol Nutr Food Res 2012; ;56:1559-68.
  • Park HJ, Jung UJ, Lee MK, Cho SJ, Jung HK, Hong JH et al. Modulation of lipid metabolism by polyphenol-rich
  • grape skin extract improves liver steatosis and adiposity in high fat fed mice. Mol Nutr Food Res 2013; 57:360-4.
  • Boqué N, de la Iglesia R, de la Garza AL, Milagro FI, Olivares M, Bañuelos O et al. Prevention of diet-induced
  • obesity by apple polyphenols in Wistar rats through regulation of adipocyte gene expression and DNA
  • methylation patterns. Mol Nutr Food Res 2013; 57:1473-8.
  • Kuem N, Song SJ, Yu R, Yun JW, Park T. Oleuropein attenuates visceral adiposity in high-fat diet-induced obese
  • mice through the modulation of WNT10b- and galanin-mediated signalings. Mol Nutr Food Res 2014; 58:2166-76.
  • Cortés-Castell E, Veciana C, Torró L, Sirvent E, Rizo-Baeza MM, Gil-Guillén VF. Actividad antiinflamatoria de un
  • extracto polifenólico de huesos de oliva en la línea celular de monocitos humanos THP1-XBLUE-CD14. Nutr
  • Hosp 2014; 30:113-7.
  • Cortés-Castell E, Veciana C, Torró L, Palazón-Bru A, Sirvent E, Gil-Guillén VF et al. Protection by polyphenol
  • extract from olive stones againts apoptosis produced by oxidative stress in human neuroblastome cells. Nutr
  • Hosp 2016; 33:118-22.
  • Cortés-Castell E, Veciana C, Torró L, Sirvent E, Rizo-Baeza MM, Gil-Guillén VF. Efecto sobre el neurodesarrollo
  • y neuroprotección en pez cebra de un extracto polifenólico de huesos de aceituna. Nutr Hosp 2014; 30:338-42.
  • Veciana C, Cortés-Castell E, Torró L, Palazón-Bru A, Sirvent E, Rizo-Baeza MM et al. Antiadipogenic activity of
  • an olive seed extract in mouse fibroblasts. Nutr Hosp 2015; 31:2747-51.
  • Veciana C, Cortés-Castell E, Torró L, Sirvent E, Rizo-Baeza MM, Gil-Guillén FV. Evaluación de la citotoxicidad y
  • bioseguridad de un extracto de polifenoles de huesos de aceitunas. Nutr Hosp 2014; 29: 1388-93.
  • Mizoguchi T, Edano T, Koshi T. A method of direct measurement for the enzymatic determination of cholesteryl
  • esters. J Lipid Res 2004; 45:396-401.
  • Nanjee MN, Miller NE. Sequential microenzymatic assay of cholesterol, triglycerides, and phospholipids in a
  • single aliquot. Clin Chem 1996; 42:915-26.
  • Coudere F. Gas chromatography/tandem mass spectrometry as an analytical tool for identification of fatty acids.
  • Lipids 1995; 30:691-9.
  • Barbazuk WB, Korf I, Kadavi C, Heyen J, Tate S, Wun E et al. The syntenic relationship of the zebrafish and
  • human genomes. Genome Res 2000; 10:1351-8.
  • Grunwald DJ, Eisen JS. Headwaters of the zebrafish -- emergence of a new model vertebrate. Nat Rev Genet
  • ; 3:717-24.
  • Lieschke GJ, Currie PD. Animal models of human disease: zebrafish swim into view. Nat Rev Genet 2007; 8:353-
  • Henderson RJ, Tocher DR. The lipid composition and biochemistry of freshwater fish. Prog Lipid Res 1987;
  • :281-347.
  • Hölttä-Vuori M, Salo VT, Nyberg L, Brackmann C, Enejder A, Panula P et al. Zebrafish: gaining popularity in lipid
  • research. Biochem J 2010; 429:235-42.
  • Song Y, Cone RD. Creation of a genetic model of obesity in a teleost. FASEB J 2007; 21:2042-9.
  • Stoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C et al. Vascular lipid accumulation, lipoprotein
  • oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish. Circ Res 2009; 104:952-60.
  • Hasumura T, Shimada Y, Kuroyanagi J, Nishimura Y, Meguro S, Takema Y et al. Green tea extract suppresses
  • adiposity and affects the expression of lipid metabolism genes in diet-induced obese zebrafish. Nutr Metab (Lond)
  • ; 9):73.
  • Flynn EJ, Trent ChM, Rawls JF. Ontogenic and nutritional control of adipogenic in zebrafish. Lipid Res 2009;
  • :1641-52.
  • Imrie D, Sadler KC. White adipose tissue development in zebrafish is regulated by both developmental time and
  • fish size. Dev Dyn 2010; 239:3013-23.
Data source: Dialnet