Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners

  1. Gallardo-Vara, Eunate 1
  2. Ruiz-Llorente, Lidia 1
  3. Bernabeu Quirante, Carmelo 1
  4. Casado-Vela, Juan 2
  5. Ruiz Rodríguez, María Jesús 3
  6. López-Andrés, Natalia 4
  7. Pattnaik, Asit K. 5
  8. Quintanilla Ávila, Miguel 6
  1. 1 (Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER))
  2. 2 (Universidad Carlos III de Madrid. Bioengineering and Aerospace Engineering Department and Centro de Investigación Biomédica en Red Enfermedades Neurodegenerativas (CIBERNED))
  3. 3 (Centro Nacional de Investigaciones Cardiovasculares (CNIC))
  4. 4 (Cardiovascular Translational Research, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA)
  5. 5 (University of Nebraska-Lincoln, School of Veterinary Medicine and Biomedical Sciences, and Nebraska Center for Virology)
  6. 6 (Instituto de Investigaciones Biomédicas "Alberto Sols", Consejo Superior de Investigaciones Científicas (CSIC), and Departamento de Bioquímica, Universidad Autónoma de Madrid (UAM))
Erakutsi afiliazioak +

Argitaratzaile: e-cienciaDatos

Argitalpen urtea: 2019

Mota: Dataset

DOI: 10.21950/ZHPOMI lock_openSarbide irekia editor

Laburpena

Co-IPs for proteomic analysis (mass spectrometry) were carried out by incubation of 1 mg of protein lysates with protein G-coated magnetic beads coupled with either the monoclonal antibody P4A4 anti-endoglin (Developmental Studies Hybridoma Bank, The University of Iowa, US) or an isotype-matched (IgG2b) control antibody (Immunostep, Salamanca, Spain). An additional control with protein G magnetic beads in the absence of antibodies was also included. After extensive washing with PBS, immunoprecipitates were then subjected to mass spectrometry analysis.