Propagación clonal in vitro de Swietenia macrophylla King (CAOBA)
- Carranza Patiño, Mercedes Susana
- Reyes Morán, Héctor
- Mora Silva, Washington
- Cevallos Falquez, Orly Fernando
- Escobar, Ariel
- Cadme Arévalo, María
- Nieto Rodríguez, Enrique
- Morante Carriel, Jaime Alfredo
ISSN: 1390-4043, 1390-4051
Year of publication: 2013
Volume: 6
Issue: 2
Pages: 1-8
Type: Article
More publications in: Revista Ciencia y Tecnología
Abstract
Mahogany (Swietenia macrophylla), is a timber tree species widely used, valued for its hardness, strength, beauty and quality. Intensive farming system and inadequate use of this species have resulted in the loss genetic variability, making impossible the implementation of breeding programs of mahogany in Ecuador. In vitro culture is a technique that would help reduce this problem by producing plants with high genetic robustness. The objective of this research was to establish a method to facilitate the in vitro mahogany propagation from nodal segments. In the establishment phase, the nodal segments were transferred at glass jars with different concentrations of Ca(ClO)2 and exposure times. Microorganism contamination was controlled with 15 g of Ca(ClO)2 for 20 min, reaching 95% survival. Healthy explants were transferred to MS/2 culture medium with different levels of benzylaminopurine (BAP) and indolebutyric acid (IBA) for their in vitro proliferation, obtaining 70% of shoots with 2 mg L-1 of BAP in combination with 1 mg L-1 IBA. The best buds of multiplication phase were placed in MS/2 culture medium with different concentrations of ANA for easy rooting, none of the treatments tested allowed to generate roots at 21 days of evaluation, although the greatest survival rate (65%) was obtained by combining 2 mg L-1 BAP + 1 mg L-1 NAA.