Efecto del precultivo con sacarosa en la criopreservación por vitrificación de plántulas de Sideritis leucantha Cav. subsp. leucantha producidas in vitro
- Jorge 1
- Ramírez-Luna, Jorge Eliseo 2
- Piqueras, Abel 3
- Casas, José Luis 2
- 1 Instituto de Investigación en Medio Ambiente y Ciencia Marina (IMEDMAR). Universidad Católica de Valencia San Vicente Mártir.
- 2 Instituto Universitario de Investigación CIBIO (Centro Iberoamericano de la Biodiversidad), Universidad de Alicante.
- 3 Departamento de Mejora Vegetal. Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC).
ISSN: 1888-8550
Año de publicación: 2021
Número: 13
Páginas: 203-210
Tipo: Artículo
Otras publicaciones en: Nereis: revista iberoamericana interdisciplinar de métodos, modelización y simulación
Resumen
Sideritis leucantha Cav. subsp. leucantha es un endemismo valenciano con uso medicinal que tiene diversos problemas de conservación. Por tanto, el objetivo principal de este trabajo fue probar el efecto de un tratamiento de precultivo para la posterior criopreservación de brotes apicales de esta especie. Los resultados mostraron que el precultivo con sacarosa 0.3 M durante 24 horas result en una mayor supervivencia de los explantes y regeneración in vitro después de tres tratamientos de vitrificación ensayados. Estos resultados sugieren que el acondicionamiento de los explantes puede mejorar la supervivencia de los explantes de S. leucantha subps. leucantha cultivados in vitro tras la criopreservación
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